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cells  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec cells
    Cells, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 230 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/foxp3+buffer+set/FoxP3+Staining+Buffer+Set/pm42401537-343-3-9
    Average 97 stars, based on 230 article reviews
    cells - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    other:

    Article Title: Method and kit for single cell protein expression profiling of floorplate mesencephalic dopaminergic progenitor cells
    Article Snippet: Therefore, cells were dissociated into single cells with TrypLE, fixed and permeabilized using the FoxP3 buffer set (Miltenyi Biotec 130-093-142).

    Article Title: Integrated scFv identification and CAR T cell generation for AML targeting in vivo
    Article Snippet: Cells were then fixed and permeabilized using the FoxP3 buffer set (Miltenyi Biotec, Bergisch Gladbach, Germany).

    Article Title: Method and kit for characterizing dopaminergic progenitor cells obtained by differentiating pluripotent stem cells
    Article Snippet: Therefore, cells were dissociated into single cells with TrypLE, fixed and permeabilized using the FoxP3 buffer set (Miltenyi Biotec 130-093-142).

    Article Title: Method and kit for characterizing dopaminergic progenitor cells obtained by differentiating pluripotent stem cells
    Article Snippet: In brief, cells were fixed and permeabilized using the FoxP3 buffer set (Miltenyi Biotec 130-093-142).

    Article Title: Method and kit for characterizing dopaminergic progenitor cells obtained by differentiating pluripotent stem cells
    Article Snippet: A sample was taken and cells were fixed and permeabilized using the FoxP3 buffer set (Miltenyi Biotec 130-093-142).

    Cell Culture:

    Article Title: Integrated scFv identification and CAR T cell generation for AML targeting in vivo.
    Article Snippet: To ensure the accuracy of these staining procedures, dead cells were effectively excluded using the 7AAD dye (BD Biosciences, USA). .. To evaluate cytokine release, CAR T cells were cultured alongside target tumor cells HL60, MV4-11, MV4-11 zsGreen at a ratio of 1:2 for 5 h in 96-well plates (Sarstedt, Germany) in the presence of 5 μg/mL Brefeldin A (Biolegend, CatNo: 42060, Netherland) and 2.0 μM Monensin (Biolegend, CatNo: 420701, Netherland) for 5 h. Cells were then fixed and permeabilized using the FoxP3 buffer set (Miltenyi Biotec, Bergisch Gladbach, Germany). .. Intracellular interferon γ (IFNγ) and tumor necrosis factor α (TNFα) were stained with anti-IFNγ-Alexa Fluor 488 (clone 4S.B3, Biolegend, Netherland) and anti-TNFα-BV421 antibodies (clone Mab11, Biolegend, San Diego, USA).

    Article Title: Integrated scFv identification and CAR T cell generation for AML targeting in vivo
    Article Snippet: To ensure the accuracy of these staining procedures, dead cells were effectively excluded using the 7AAD dye (BD Biosciences, USA). .. To evaluate cytokine release, CAR T cells were cultured alongside target tumor cells HL60, MV4‐11, MV4‐11 zsGreen at a ratio of 1:2 for 5 h in 96‐well plates (Sarstedt, Germany) in the presence of 5 μg/mL Brefeldin A (Biolegend, CatNo: 42060, Netherland) and 2.0 μM Monensin (Biolegend, CatNo: 420701, Netherland) for 5 h. Cells were then fixed and permeabilized using the FoxP3 buffer set (Miltenyi Biotec, Bergisch Gladbach, Germany). .. Intracellular interferon γ (IFNγ) and tumor necrosis factor α (TNFα) were stained with anti‐IFNγ‐Alexa Fluor 488 (clone 4S.B3, Biolegend, Netherland) and anti‐TNFα‐BV421 antibodies (clone Mab11, Biolegend, San Diego, USA).

    Staining:

    Article Title: Ferroptosis-armed dendritic cell vaccines for glioma immunotherapy.
    Article Snippet: .. For intracellular FoxP3 staining, cells were fixed and permeabilized using the FOXP3 buffer set (Miltenyi Biotech, Cat. 130-093-142) according to the manufacturer’s instructions. .. Samples were acquired using a BD Symphony A5 flow cytometer (BD, USA), and data were analyzed with FlowJo software (V.10.0.8).



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